Description
| Source | Applications | Cross-reactivity | Theoretical | Actual M.W. |
| Mouse | WB, IHC, ICC | All | ~50kDa | 50-55kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
A Primary Antibody Dilution Buffer is provided with this product and can be used for primary antibody dilution in Western blot or other applications.
The recommended dilution factors of this product in different applications are as follows. They can be adjusted appropriately according to the level of antigen in samples.
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
The detailed information is as follows:
| About this Antibody | |
| Name | β-Tubulin Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purification method | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide ESNMNDLVSEYQQYQDATAC coupled to KLH |
| Sequence | ESNMNDLVSEYQQYQDATAC |
| Gene ID | 203068 |
| SwissProt | P07437 |
| Synonyms | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 |
| Category | Cytoskeletal Signaling |
| Background | Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β-tubulin. Our anti-β-tubulin monoclonal antibody provides a specific and useful tool in studying the intracellular distribution of tubulin and the static and dynamic aspects of the cytoskeleton. |
| Item | Component | Quantity |
| AF2839-50µl | β-Tubulin Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ200 | Primary Antibody Dilution Buffer for Western Blot | 50ml |
| Manual | – | 1 copy |
Store the Antibody at -20℃ and Primary Antibody Dilution Buffer at 4℃ for up to one year. The Primary Antibody Dilution Buffer can be stored at -20℃ for lone-term storage, but freeze-thaw may result in slight turbidity and a small amount of insoluble substance.
This product can be recovered for multiple reuses (5-10 times) in Western blot (WB), immunofluorescence (IF), and immunocytochemistry (ICC). Store the unused or recovered diluted antibodies at 4℃.
The recovered antibodies can be reused in the same way as freshly diluted antibodies. If slight turbidity appears, centrifuge the antibody at 10,000×g for 1-3 minutes and use the supernatant for subsequent assays. But it should be discarded when there is significant flocculation or any microbial growth.
The Primary Antibody Dilution Buffer included in this product can also be used in immunofluorescence (IF), immunohistochemistry (IHC), immunocytochemistry (ICC), and other appropriate applications. But for optimal results, we recommend using the primary antibody dilution buffer specifically designed for each assay.
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
