Description
AF555-labeled Donkey Anti-Rabbit IgG (H+L) is a secondary antibody for immunofluorescence staining.
Alexa Fluor (AF) dyes, trademarked by Molecular Probes, are advanced fluorescent dyes derived from Coumarin, Rhodamine, Xanthene, and Cyanine via sulfonation and chemical modification (e.g., Alexa Fluor 488, or AF488). They feature a versatile excitation/emission range (visible to infrared, blue to red), hydrophilic and stable properties (water-soluble, non-precipitating conjugates), enhanced performance (better photostability and signal strength for low-abundance targets), biocompatibility (low toxicity), and pH resilience (strong fluorescence across pH 4-10) [1]. They are ideal for labeling antibodies and proteins in fluorescence microscopy, flow cytometry, and fluorescence-based assays.
AF555 is a commonly used probe exhibiting bright red fluorescence (Ex/Em = 555nm/565nm) that is brighter, less prone to quenching, and produces a lower background, compared to most other red fluorescent probes. Its fluorescence spectrum is close to Cy3.
| Fluorophore |
Absorption Peak (nm) |
Emission Peak (nm) |
| AF555 |
555 |
565 |
This high-quality secondary antibody is obtained by immunizing donkeys with purified rabbit IgG, followed by affinity purification and adsorption to human, mouse, and rat IgG.
The recommended dilution factor of this product for immunofluorescence staining is 1:500. However, the dilution factor can be optimized for different antigens and antibodies between 1:200-1:1000.
This product allows for 50 assays when 1 ml of this product diluted at 1:500 is used for each assay, and 150-250 additional assays if the diluted antibody will be reused.
Packing List:
| Item |
Component |
Quantity |
| A0453 |
AF555-labeled Donkey Anti-Rabbit IgG (H+L) |
100μl |
| Manual |
– |
1 copy |
Storage Conditions:
Store at -20°C in the dark, stable for up to 1 year.
Precautions:
This product is for research use only, not for drug, clinical diagnosis, food, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
References
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