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Alexa Fluor 555-labeled Donkey Anti-Mouse IgG(H+L)

Alexa Fluor 555-labeled Donkey Anti-Mouse IgG(H+L)

€ 120,00

AF555-labeled Donkey Anti-Mouse IgG (H+L) is a secondary antibody for immunofluorescence staining.

Package: 100ul

SKU: A0460
Category: , , ,

Description

AF555-labeled Donkey Anti-Mouse IgG (H+L) is a secondary antibody for immunofluorescence staining.

Alexa Fluor (AF) dyes, trademarked by Molecular Probes, are advanced fluorescent dyes derived from Coumarin, Rhodamine, Xanthene, and Cyanine via sulfonation and chemical modification (e.g., Alexa Fluor 488, or AF488). They feature a versatile excitation/emission range (visible to infrared, blue to red), hydrophilic and stable properties (water-soluble, non-precipitating conjugates), enhanced performance (better photostability and signal strength for low-abundance targets), biocompatibility (low toxicity), and pH resilience (strong fluorescence at pH 4-10) [1]. They are ideal for labeling antibodies and proteins in fluorescence microscopy, flow cytometry, and fluorescence-based assays.

AF555 is a commonly used probe exhibiting bright red fluorescence (Ex/Em = 555nm/565nm) that is brighter, less prone to quenching, and produces a lower background compared to most other red fluorescent probes. Its fluorescence spectrum is close to Cy3.

Fluorophore Absorption Peak (nm) Emission Peak (nm)
AF555 555 565

This high-quality secondary antibody is obtained by immunizing donkeys with purified mouse IgG, followed by affinity purification and adsorption to human, rabbit, and rat IgG.

The recommended dilution factor of this product for immunofluorescence staining is 1:500. However, the dilution factor can be optimized for different antigens and antibodies between 1:200-1:1000.

This product allows for 50 assays when 1 ml of this product diluted at 1:500 is used for each assay, and 150-250 additional assays if the diluted antibody will be reused.

Packing List

Item Component Quantity
A0460 AF555-labeled Donkey Anti-Mouse IgG (H+L) 100μl
Manual – 1 copy

Storage Conditions

Store at -20°C in the dark, stable for up to 1 year.

Precautions

This product is for research use only, not for drug, clinical diagnosis, food, or other uses.

For your safety and health, please wear a lab coat and disposable gloves during the operation.

Additional information

Weight 2 kg
Dimensions 20 × 20 × 20 cm

Instruction

Instructions for Use：

1. For immunofluorescence staining, please refer to the relevant experimental procedures. The recommended dilution factor of this product in this manual can be used as a start.
2. To reuse the diluted fluorescence-labeled secondary antibody, store it at 4℃.

Product files

MSDS

https://scandicbiotech.com/wp-content/uploads/2026/04/A0460-1012.pdf

References

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