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Flag Mouse Monoclonal Antibody

Flag Mouse Monoclonal Antibody

€ 138,00

This high-purity Flag monoclonal antibody, clone number M2, is generated by using the modified synthetic Flag peptide (DYKDDDDK) as the antigen, followed by a two-step purification using protein G and antigen affinity chromatography.

Package: 40ul

SKU: AF519
Category: , , ,

Description

Source Applications Recognition Site Antibody Type
Mouse WB, IP, IF Flag tag IgG1

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence.

This high-purity Flag monoclonal antibody, clone number M2, is generated by using the modified synthetic Flag peptide (DYKDDDDK) as the antigen, followed by a two-step purification using protein G and antigen affinity chromatography.

This product is widely used to analyze Flag fusion proteins. It can detect Flag tag located at the N-terminus or C-terminus of fusion proteins, as well as Flag tag located inside the fusion protein.

This product can be used to purify Flag tag fusion proteins, examine their subcellular location, and determine their expression levels by qualitative or quantitative analysis.

A Primary Antibody Dilution Buffer is provided with this product and can be used for primary antibody dilution in Western blot or other applications.

The recommended dilution factors of this product in different applications are as follows. They can be adjusted appropriately according to the level of antigen in samples.

WB IP IF
1:1000 1:100 1:1000

 

When using for routine Western blot, this product is sufficient for at least 40 blots.

Packing List：
Item Component Quantity
AF519-1 Flag Mouse Monoclonal Antibody 40µl
AF519-2 Primary Antibody Dilution Buffer for Western Blot 40ml
Manual – 1 copy

 

Storage Conditions：

Store the Antibody at -20℃ and Primary Antibody Dilution Buffer at 4℃ for up to one year. The Primary Antibody Dilution Buffer can be stored at -20℃ for lone-term storage, but freeze-thaw may result in slight turbidity and a small amount of insoluble substance.

Precautions：

This product can be recovered for multiple reuses (at least 10 times) in Western blot (WB). Store the unused or recovered diluted antibodies at 4℃.

The recovered antibodies can be reused in the same way as freshly diluted antibodies. If slight turbidity appears, centrifuge the antibody at 10,000×g for 1-3 minutes and use the supernatant for subsequent assays. But it should be discarded when there is significant flocculation or any microbial growth.

This product is for R&D only. Not for drug, household, or other uses.

For your safety and health, please wear a lab coat and disposable gloves during the operation.

 

Additional information

Weight 2 kg
Dimensions 20 × 20 × 20 cm

Instruction

Instructions for Use：

1. Western blot
a. Dilute an appropriate amount of the antibody with the provided Primary Antibody Dilution Buffer to an appropriate concentration as required.
b. Transfer the blocked membrane into the diluted primary antibody and incubate at room temperature for 2 hours or overnight at 4℃ with gently shaking. The membrane should be covered completely by the diluted antibody.
c. Recover the diluted primary antibody and store at 4℃ for later use.
d. Proceed to next step in Western blot. For detailed procedures of Western blot, please refer to http://www.beyotime.com/support/western.htm.
2. Immunostaining
For Immunostaining, we recommend using the Immunol Staining Primary Antibody Dilution Buffer (Beyotime, P0103) to dilute the antibody. The diluted antibody can be recovered for multiple reuses. For detailed procedures of Immunostaining, please refer to http://www.beyotime.com/support/immunol-staining.htm.
3. For other applications, please refer to appropriate protocols.

Product files

MSDS

https://scandicbiotech.com/wp-content/uploads/2026/04/AF519-A1033.pdf

References

Related papers：

1. Xu W, Wang S, Chen Q, Zhang Y, Ni P, Wu X, Zhang J, Qiang F, Li A, R?e OD, Xu S, Wang M, Zhang R, Zhou J.
.
CELL DEATH DIS. 2014 Feb 13;5:e1055. (IF 6.304)
2. Zhang K, Fu G, Pan G, Li C, Shen L, Hu R, Zhu S, Chen Y, Cui H
.
Cell Death Dis. 2018 Oct 10;9(10):1035. (IF 6.304)
3. Lu Q, Li N, Luo J, Yu M, Huang Y, Wu X, Wu H, Liu XY, Li G.
.
Oncogenesis. 2012 Oct 15;1:e29. (IF 6.119)
4. Ren HY, Wang J, Yang F, Zhang XL, Wang AL, Sun LL, Diao KX, Wang EH, Mi XY.
.
ONCOTARGET. 2015 Feb 28;6(6):4080-96. (IF 5.168)
5. Li K, Lu Y, Liang J, Luo G, Ren G, Wang X, Fan D.
.
BIOCHEM BIOPH RES CO. 2009 Feb 6;379(2):212-6. (IF 2.985)
6. Zhao J, Yu H, Lin L, Tu J, Cai L, Chen Y, Zhong F, Lin C, He F, Yang P.
.
J Proteomics. 2011 Dec 21;75(2):588-602. (IF 3.509)
7. Bao J, Wang L, Lei J, Hu Y, Liu Y, Shen H, Yan W, Xu C.
.
Histochem Cell Biol. 2012 Mar;137(3):377-89. (IF 3.418)
8. Yan A, Li G, Zhang X, Zhu B, Linghu H.
.
MED SCI MONITOR. 2013 Jan 14;19(1):12-9. (IF 1.918)
9. Zeng Z, Zhang C, Chen J.
.
J Bone Miner Metab. 2013 Jul;31(4):409-16. (IF 2.297)
10. Zhang X, Li G, Zhang L, Yang D, Zhang Z, Yan A, Linghu H.
.
Cell Biol Int. 2013 Oct;37(10):1106-13. (IF 2.571)
11. Wang J, Su H, Zhang T, Du J, Cui S, Yang F, Jin Q.
.
PLoS One. 2014 Mar 24;9(3):e92565. (IF 2.74)
12. Wang J, Du J, Jin Q.
.
PLoS One. 2014 Jun 9;9(6):e99768. (IF 2.74)
13. Liu Y, Ma S, Wang X, Chang J, Gao J, Shi R, Zhang J, Lu W, Liu Y, Zhao P, Xia Q.
.
INSECT BIOCHEM MOLEC. 2014 Jun;49:35-42. (IF 3.827)
14. Miao SH, Sun HB, Ye Y, Yang JJ, Shi YW, Lu M, Hu G, Zhou JW.
.
CNS Neurosci Ther. 2014 Aug;20(8):754-62. (IF 4.074)
15. Xu Z, Liu D, Fan C, Luan L, Zhang X, Wang E.
.
MOL CARCINOGEN. 2014 Nov;53(11):917-25. (IF 3.825)
16. Zhao D, Huang X, Liu Y, Han K, Zhang J, Yang J, Xie X, Li Y.
.
Res Vet Sci. 2015 Feb;98:121-6. (IF 1.892)
17. Zhang ZS, Yang DY, Fu YB, Zhang L, Zhao QP, Li G.
.
Cell Biochem Funct. 2015 Mar;33(2):80-8. (IF 2.632)
19. Cai R, Xue W, Liu S, Petersen RB, Huang K, Zheng L.
.
FASEB J. 2015 Jul;29(7):2749-58. (IF 4.966)
20. Wang Y, Yan X, Zeng S, Zhang T, Cheng F, Chen R, Duan C
.
Biotechnol Lett. 2018 Jun 19. (IF 1.977)
21. Ji Y, Jiang J, Huang L, Feng W, Zhang Z, Jin L, Xing X
.
Oncol Rep. 2018 Aug;40(2):783-792. (IF 3.417)
22. Yang D, Zhang L, Zhang Z, Hu S, Fu Y, Laukkanen JA, Li G
.
CLIN EXP PHARMACOL P . 2019 Mar;46(3):237-245. (IF 2.092)
23. Qi Huang, Xue-Mei Li, Jing-Ping Sun, Yan Zhou
.
Cancer Med. 2023 Feb;12(3):3222-3236. doi: 10.1002/cam4.5055.
24. Huiping Zhao, Zhongyuan Ge, Mengmeng Zhou, Hongqiu Zeng, Yunxie Wei, Guoyin Liu, Yu Yan, Russel J Reiter, Chaozu He, Haitao Shi
.
J Pineal Res. 2023 Apr;74(3):e12861. doi: 10.1111/jpi.12861.
25. Weilu Zhang, Shudai Lin, Zhenhai Jiao, Lilong An, Tingting Xie, Jiang Wu, Li Zhang
.
Genes (Basel). 2023 May 31;14(6):1207. doi: 10.3390/genes14061207.
26. Yan Zhao, Qiang Luo, Wansu Wang, Shang Geng, Yuena Sun, Tianjun Xu
.
Dev Comp Immunol. 2023 Aug:145:104713. doi: 10.1016/j.dci.2023.104713.
27. Yingjie Li, Jiaming Xu, Yan Xu, Chuanzhou Li, Yan Wu, Zhijun Liu
.
Ann Clin Transl Neurol. 2023 Sep;10(9):1556-1568. doi: 10.1002/acn3.51845.
28. Xiaowen Chen, Xin Hou, Hao Yang, Hao Liu, Jun Wang, Chenghui Wang
.
Front Endocrinol (Lausanne). 2023 Oct 19:14:1251723. doi: 10.3389/fendo.2023.1251723.
29. Qiang Luo, Xuefeng Zhou, Xing Lv, Weiwei Zheng, Shang Geng, Tianjun Xu, Yuena Sun
.
Fish Shellfish Immunol. 2023 Nov:142:109147. doi: 10.1016/j.fsi.2023.109147.

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