Description
This product is molecular biology grade, free of DNase and RNase, and can be used as a carrier to increase the recovery of nucleic acids from diluted solutions by alcohol precipitation.
As a precipitant for nucleic acids, glycogen works better than tRNA or sonicated DNA in most cases. Since glycogen does not contain DNA and RNA, the nucleic acids precipitated with glycogen is more suitable for subsequent PCR, RT-PCR, and endonuclease reactions.
It was reported that precipitation of ligation products with glycogen has no interference with subsequent bacterial transformations. Glycogen of 0.001mg/ml will not inhibit TdT, glycogen of no more than 2mg/ml will not affect the activity of reverse transcriptase, and 0.02mg/ml glycogen will not inhibit T4 RNA ligase. However, glycogen interferes with DNA-protein interactions.
Usually, 1μl of Glycogen (20mg/ml) can precipitate at least picogram (pg) of DNA or RNA from 1ml of solution. Each package of this product is sufficient to precipitate 500 DNA or RNA samples in conventional quantities.
| Cat. No. | Product Name | Pack Size |
| D0812 | Glycogen (20mg/ml) | 500μl |
| – | Information Sheet | 1 copy |
Store at -20℃, valid for at least 1 year.
Usually, 1μl of Glycogen (20mg/ml) is sufficient for each sample. If glycogen may interfere with subsequent reactions, the amount of glycogen can be appropriately reduced, or tRNA can be used as a precipitant instead.
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
