Description
Cy3-labeled Donkey Anti-Goat IgG (H+L) is a secondary antibody for immunofluorescence staining.
Cy3 is a newly developed probe exhibiting red fluorescence (Ex/Em = 550nm/570nm) that is brighter, less prone to quenching, and produces a lower background than most other red fluorescent probes.
| Fluorophore |
Absorption Peak (nm) |
Emission Peak (nm) |
| Cy3 |
550 |
570 |
This high-quality secondary antibody is obtained by immunizing donkeys with purified goat IgG, followed by affinity purification from antiserum and adsorption to mouse, rat, rabbit, bovine, and human IgG to ensure minimal cross-reactivity with mouse, rat, rabbit, bovine, and human serum proteins.
The recommended dilution factor of this product for immunofluorescence staining is 1:500. However, the dilution factor can be optimized for different antigens and antibodies between 1:200-1:1000.
This product allows for 25 assays when 1 ml of this product diluted at 1:500 is used for each assay, and 75-125 additional assays if the diluted antibody will be reused.
Packing List
| Item |
Component |
Quantity |
| A0502 |
Cy3-labeled Donkey Anti-Goat IgG (H+L) |
50μl |
| Manual |
– |
1 copy |
Storage Conditions
Store at -20°C in the dark, stable for up to 1 year.
Precautions
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
References
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