Description
| Source |
Applications |
WB |
ELISA |
IHC |
| Goat |
WB, ELISA, IHC |
1:500-5000 |
1:500-5000 |
1:500-2000 |
WB, Western blot; IHC, Immunohistochemistry.
AP-labeled Goat Anti-Rabbit IgG (H+L) is a high-purity and high-specificity secondary antibody for Western, ELISA, IHC, etc.
Alkaline phosphatase (AP/ALP/AKP/ALKP/ALPase/Alk Phos, EC 3.1.3.1) is a class of hydrolases that remove phosphate groups from substrates by hydrolyzing phosphate monoesters and generating phosphate ions and free hydroxyl groups. The substrates include proteins, nucleotides and alkaloids. This product is more effective under alkaline conditions. The Calf Intestinal Alkaline Phosphatase (CIAP/CIP) is widely used for labeling of secondary antibodies to detect proteins and nucleic acids in Western blot, ELISA, IHC, ICC, EMSA, Northern blot, and Southern blot, etc. This product is labeled with the CIAP that can catalyze the color development of chromogenic reagents such as BCIP/NBT or the production of chemiluminescence with appropriate chemiluminescent reagents.
This high-quality secondary antibody is obtained by immunizing goats with purified rabbit IgG, followed by affinity purification from antiserum and adsorption to human, mouse, and rat IgG. It has minimal cross-reactivity to human, mouse, and rat serum proteins.
The recommended dilution factor of this product for WB, ELISA and IHC are shown in the table above. However, the dilution factor can be optimized for different antigens and antibodies.
This product allows for 50 routine Western blots when 10 ml of this product diluted at 1:1000 is used for each blot.
Packing List:
| Item |
Component |
Quantity |
| A0239 |
AP-labeled Goat Anti-Rabbit IgG (H+L) |
0.5ml |
| Manual |
– |
1 copy |
Storage Conditions:
Store at -20 °C, stable for up to 1 year.
Precautions:
- This product is for R&D only. Not for drug, household, or other uses.
- For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instruction
Instructions for Use：
1. Please refer to relevant experimental procedures for Western blot, ELISA, and IHC assays. The recommended dilution factor of this product in this manual for different applications can be used as a start.
References
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